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背景介绍 Mesothelin (MSLN) is a glycophosphatidylinositol (GPI) linked cell-surface protein that is produced as a ~70 kDa precursor protein and cleaved by Furin protease to generate the ~40 kDa mature form. MSLN is frequently over-expressed in mesothelioma, ovarian, pancreatic, and non-small cell lung cancers, while its expression in normal tissues is restricted to the mesothelial lining. MSLN is a tumor-associated antigen and has been an attractive target for targeted immunotherapy approaches, including drug-conjugated antibodies and chimeric antigen receptor T-cells (CAR-T Cells). 产品介绍 Recombinant CHO-K1 cells expressing cleaved, mature human Mesothelin (MSLN) gene (GenBank Accession #NM_005823.5) under the control of cytomegalovirus (CMV) promoter. This cell line was generated by limited dilution and isolation of individual clones, which were screened based on MSLN expression to obtain a high-expressing cell line.
询价背景介绍 RANK/RANKL/OPG pathway plays a significant role in osteoclast maturation, which are the major cells in charge of bone resorption. If RANKL binds to the receptor RANK instead of binding to its decoy receptor Osteoprotegerin (OPG), it activates the NF-κB pathway to promote osteoclast survival and proliferation, resulting in the resorption of bone. Therefore, abnormal production of RANKL or OPG can cause osteoporosis and other bone-related disorders, making the RANKL/RANK signaling pathway a valuable target for drug development. 产品介绍 Recombinant HEK-293 cells expressing firefly luciferase gene under the control of Nuclear factor-κB (NF-κB) with constitMedium 1Gutive expression of human RANK (Receptor activator of nuclear factor-κB; TNFRSF11A; ref. seq. NM_003839.2).
询价背景介绍 GPC3, also known as Glypican-3 and OCI5, belongs to the glypican family and is highly expressed in the lungs, liver and kidneys. Its function is tissue dependent and can either promote or suppress tumorigenesis. Being a heparin sulfate proteoglycan, it is overexpressed in neoplasms including malignant melanoma, hepatocellular carcinoma, and testicular yolk sac tumors and plays a significant role in cell growth and differentiation. 产品介绍 Recombinant CHO-K1 cells expressing GPC3 (Glypican 3), a cell surface proteoglycan bearing heparin-sulfate; ref. seq. NM_004484.2.
询价背景介绍 Trophoblast cell-surface antigen 2 (TROP-2), also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein that is highly expressed in a variety of solid cancers, yet has low expression in normal cells. Through a variety of signaling pathways, TROP2 regulates cancer growth and metastasis and is a favorable target for antibody drug conjugates (ADC) and immunotherapy. 产品介绍 Recombinant CHO-K1 cells expressing the cell surface glycoprotein TROP2 (Trophoblast cell-surface antigen 2); ref. seq. NM_002353.2.
询价产品介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Daudi cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
询价产品介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing Neuro2A cell pool can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
询价背景介绍 Human transmembrane serine protease 2 (TMPRSS2) is an enzyme primarily expressed by endothelial cells across the respiratory and digestive tracts. It is involved in viral entry and spread of coronaviruses including SARS-CoV-2, the virus that causes COVID19. Blocking TMPRSS2 could potentially be an effective clinical therapy for COVID-19. 产品介绍 Recombinant clonal stable Vero E6 cell line constitutively expressing full length human TMPRSS2, Genbank #NM_005656.4.
询价产品介绍 This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2-tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.
询价产品介绍 Cas9 (Streptococcus pyogenes CRISPR associated protein 9) is an endonuclease enzyme that, when recruited to a specific DNA sequence by the sgRNA (single guide RNA), introduces a double stranded break into the DNA. This double stranded break is repaired in mammalian cells either through Non-Homologous End Joining or Homologous Recombination. Non-Homologous End Joining often results in the deletion or insertion of several base pairs at the cut site, which, when resulting in a frameshift, causes the functional inactivation of the targeted gene. Cas9-expressing HCT116 cells can be transduced or electroporated with sgRNA targeting a gene of interest to quickly generate knock-out cell pools or cell lines.
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