服务平台拥有丰富的技术服务经验，一流的仪器设备，先进的检测手段和严格的质量控制体系，力求为全球药物研发机构提供优质高效的技术服务
背景介绍 The transforming growth factor beta (TGFβ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGFβ signaling has been linked to cardiac disease, cancer, Alzheimer's and other human diseases. TGFβ proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGFβ/ SMAD responsive genes. 产品介绍 The SBE Reporter - HEK293 Cell Line is designed for monitoring the activity of the TGF /SMAD signaling pathway. The transforming growth factor beta (TGF ) signaling pathway is involved in a diverse range of cell processes such as differentiation, cell cycle arrest, and immune regulation. TGF signaling has been linked to cardiac disease, cancer, Alzheimer's and other human diseases. TGF proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to phosphorylation and activation of SMAD2 and SMAD3, which then form a complex with SMAD4. The SMAD complex then translocates to the nucleus and binds to the SMAD binding element (SBE) in the nucleus, leading to transcription and expression of TGF / SMAD responsive genes.
询价背景介绍 The notch signaling pathway is important for cell-cell communication, which involves gene regulation mechanisms that control multiple cell differentiation processes during embryonic and adult life. Notch signaling also has a role in neuronal function, embryonic development, angiogenesis, cardiac homeostasis, and bone regeneration. Notch signaling is dysregulated in many cancers, and faulty notch signaling is implicated in many diseases including T-ALL (T-cell acute lymphoblastic leukemia) and Multiple Sclerosis. 产品介绍 The Notch CSL Reporter - HEK293 cell line contains the firefly luciferase gene under the control of Notch-response elements (CSL responsive elements) alone with expression construct for Notch1 E (NOTCH1 that has a deletion of the entire extracellular domain) stably integrated into HEK293 cells. Inside the cells, the Notch1 E can be cleaved by γ-secretase. The active Notch1 NICD is released to nucleus and induces the constitutive expression of luciferase reporter. The cell line is validated for the inhibition of the expression of luciferase reporter using a known inhibitor of the Notch signaling pathway.
询价产品介绍 NF-κB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
询价背景介绍 Nuclear factor-κB (NF-κB)/Rel proteins include NF-κB2 p52/p100, NF-κB1 p50/p105, c-Rel, RelA/p65, and RelB. These proteins function as dimeric transcription factors that control genes regulating a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B cell development, and lymphoid organogenesis. In the classical (or canonical) pathway, NF-κB/Rel proteins are bound and inhibited by IκB proteins. Proinflammatory cytokines, LPS, growth factors, and antigen receptors activate an IKK complex (IKKβ, IKKα, and NEMO), which phosphorylates IκB proteins. Phosphorylation of IκB leads to its ubiquitination and proteasomal degradation, freeing NF-κB/Rel complexes. Active NF-κB/Rel complexes are further activated by phosphorylation and translocate to the nucleus where they induce target gene expression. In the alternative (or noncanonical) NF-κB pathway, NF-κB2 p100/ RelB complexes are inactive in the cytoplasm. Signaling through a subset of receptors, including LTβR, CD40, and BR3, activates the kinase NIK, which in turn activates IKKα complexes that phosphorylate C-terminal residues in NF-κB2 p100. Phosphorylation of NF-κB2 p100 leads to its ubiquitination and proteasomal processing to NF-κB2 p52, creating transcriptionally competent NF-κB p52/RelB complexes that translocate to the nucleus and induce target gene expression. 产品介绍 The NF-κB reporter (luc)-HEK293 cell line is designed for monitoring the nuclear factor Kappa B (NF-κB) signal transduction pathways. It contains a firefly luciferase gene driven by four copies of NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene.
询价背景介绍 Foxp3, belonging to the forkhead family, is a master transcription factor that expresses exclusively in regulatory T cells, a subset of CD4+ T cells. Regulation of Foxp3 is critical for maintaining immunological tolerance. Over-expression of Foxp3 is known to suppress effector T cell activation. 产品介绍 Human Foxp3 luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by a human Foxp3 promoter and an enhancer-like conserved noncoding sequence upstream of the Foxp3 promoter.
询价背景介绍 CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. Antigen presenting cells (APCs) such as monocytes, basophils, dendritic cells, have been found to express CD40. Additionally, CD40 has been identified in non-immune cells such as endothelial cells and epithelial cells. A wide variety of carcinoma cells over-express CD40 as well. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Furthermore, agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC) and promote anti-tumor T-cell responses in addition to fostering cytotoxic myeloid cells with the potential to control cancer in the absence of T-cell immunity. 产品介绍 Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5). Expression is confirmed by real-time qPCR and Western Blot. This NF-κB luciferase reporter construct is stably integrated into the genome. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by human CD40 ligand, NF-κB transcription factor binds to the DNA response elements to induce transcription of the luciferase gene.
询价背景介绍 NF-κB signaling plays a pivotal role in regulating cell development and immune homeostasis. Activation of NF-κB through tumor necrosis factor receptors (TNFR) or the TNFR superfamily member CD40 occurs upon engagement with their respective ligands TNFα or CD40L. Activation of NF-κB enhances cell inflammation and prevents apoptosis, which contribute to tumor development. The A549 lung epithelial cell line is ideal in an in vitro lung disease model for high throughput screening of oncogene inhibitors upstream of the NF-κB signaling pathway. 产品介绍 NF-κB luciferase reporter construct is stably integrated into the genome of A549 cells. The firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
询价背景介绍
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the
T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is
expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF-
_x0001_B and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed
by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27
signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the
induction of long-term memory. The CD27/CD70 pathway is a key target for the development of
treatments for cancer and inflammatory diseases.
产品介绍
CHO-K1 cell line expressing full length human CD27 (TNFRSF7). Expression is confirmed by real time qPCR and Western blot.
Screening for antibodies recognizing CD27 and screening antibodies for binding affinities. Since
CHO-K1 cells do not express endogenous CD27, this cell line serves as an excellent model for
studying changes in CD27-mediated signaling pathways, such as NF-B, MAPK and
SAPK/JNK.
产品介绍
NF-_x0001_B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene.
The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).
