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背景介绍 The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizes with Max to regulate transcription of genes involved in proliferation, transformation and angiogenesis. Myc mutations have been linked to the development of a number of human cancers, including Burkitt's lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma, making Myc a promising therapeutic target. 产品介绍 The Myc Reporter - HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into HCT116 cells, a human colon cancer cell line. HCT116 contains a mutated _x0001_beta-catenin which leads to the accumulation of β-catenin and constitutive activation of downstream Myc that induces the expression of Myc luciferase reporter. The cell line is validated for the inhibition of the expression of Myc luciferase reporter.
询价背景介绍 The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. It is the main binding site of cAMP response element binding protein (CREB) and is responsible for its activation. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms. In the cAMP/PKA signaling pathway, CREB is activated via phosphorylation of PKA and binds to CRE with a general motif of 5'-TGACGTCA-3'. Since CRE is a modulator of the cAMP/PKA signaling pathway, it allows the effects of various inhibitors to be studied. 产品介绍 The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) - HEK293 Cell Line is designed for monitoring the activity of the cAMP/ PKA signaling pathway. The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into HEK293 cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase.
询价背景介绍 The Nrf2 antioxidant response pathway plays an important role in cellular antioxidant defense. Nrf2, a basic leucine zipper transcription factor, induces the expression of antioxidant and phase II enzymes by binding to the ARE (antioxidant response element) region of the gene promoter. Under basal conditions, Nrf2 is retained in the cytosol by binding to the cytoskeletal protein Keap1. Upon exposure to oxidative stress or other ARE activators, Nrf2 is released from Keap1 and translocates to the nucleus, where it can bind to the ARE, leading to the expression of antioxidant and phase II enzymes that protect the cell from oxidative damage. 产品介绍 The ARE Reporter - Hep G2 cell line is designed to monitor Nrf2 antioxidant response pathway. The cell contains a firefly luciferase gene under the control of ARE (antioxidant response element) stably integrated into Hep G2 cells.
询价背景介绍 The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Binding of Interferon alpha (IFNα) to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex known as ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes. 产品介绍 The ISRE Reporter - HEK293 Cell Line is designed for monitoring the activity of the JAK/STAT signaling pathway. The ISRE Reporter - HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells.
询价背景介绍 Nav1.7 is a voltage-gated sodium ion channel that in humans is encoded by the SCN9A gene. It is usually expressed at high levels in two types of neurons, the nociceptive neurons at dorsal root ganglion and trigeminal ganglion, and sympathetic ganglion neurons, which are part of the autonomic (involuntary) nervous system. Nav1.7 is present at the endings of pain-sensing nerves, the nociceptors, close to the region where the impulse is initiated.The Nav1.7 channel produces a rapidly activating and inactivating current which is sensitive to the level of tetrodotoxin. Knockout mice that lack Nav1.7 in nociceptors showed reduced response to inflammatory pain. 产品介绍 Stable recombinant HEK293 cell line expressing human Nav1.7 (Genbank # Q15858) fused at the C-terminus to Green Fluorescent Protein [Ex. ~395 nm, 475 nm; em ~510 nm] and with Cterminal Streptavidin-Binding Peptide (SBP) fusion. Nav1.7 is a tetrodotoxin-sensitive voltagegated sodium channel type IX subunit alpha (SCN9A).
询价背景介绍 Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes. 产品介绍 The CFTR-HEK293 Cell Line stably expresses full length, human cystic fibrosis transmembrane conductance regulator (CFTR) protein (GenBank # P13569). A synthetic codon-optimized DNA sequence encoding human CFTR protein with C-terminal Streptavidin-Binding Peptide (SBP) tag is stably integrated in 293HEK-Trex cells.
询价背景介绍 RAR belongs to a family of nuclear receptors and has three subtypes, RARα, RARβ, and RARγ. RAR heterodimerizes with RXR (retinoid X receptor) and acts as a transcription factor that regulates the growth and differentiation of both normal and malignant cells. When RAR binds to its ligands, all-trans retinoic acid or 9-cis retinoic acid, RAR/RXR heterodimer binds to retinoic acid response elements in the promoter region of target genes and recruits coactivator proteins, leading to transcription and expression of the downstream target genes. 产品介绍 The RARα Reporter (Luc)-HEK293 Cell Line contains a firefly luciferase gene under the control of retinoic acid response elements stably integrated into HEK293 cells along with full length human RARα (NM_000964).
询价背景介绍 The Wnt pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. Wnt proteins bind to receptors on the cell surface, initiating a signaling cascade that leads to stabilization and nuclear translocation of beta-catenin. beta-catenin then binds to TCF/LEF transcription factors in the nucleus, leading to transcription and expression of Wnt-responsive genes. 产品介绍 The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line is designed for monitoring the activity of the Wnt/b-catenin signaling pathway. The Wnt Signaling Pathway TCF/LEF Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of TCF/LEF responsive elements stably integrated into HEK293 cells. This cell line is validated for the response to the stimulation of mouse Wnt3a and to the treatment with an inhibitor of Wnt/b-catenin signaling pathway.
询价背景介绍 Glucocorticoid-induced TNFR-related protein (GITR) is a member of the TNFR superfamily, expressed in a number of cells including T cells, natural killer (NK) cells and antigen-presenting cells (APC). GITRL, GITR's natural ligand, is expressed mainly by APCs and GITR:GITRL interaction is important for activation of the immune system. Agonistic antibodies targeting GITR are actively being pursued as potential immuno-oncology therapies. 产品介绍 Recombinant HEK293 cell expressing firefly luciferase gene under the control of NF-κB response elements with constitutive expression of human OX40 (Tumor Necrosis Factor Receptor Superfamily Member 4, TNFRSF4, and CD134, GenBank Accession No. NM_003327)
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