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背景介绍 Interleukin-2 (IL-2) is a key cytokine important for proliferation and differentiation of T cells into effector T cells through interaction with the IL-2 receptor. 产品介绍 Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.
询价产品介绍 The PDE7A/CRE Reporter-HEK293 line contains the firefly luciferase gene under the control of CRE as well as a constitutive expression construct for human PDE7A (phosphodiesterase 7A, accession number NM_002603), both stably integrated into HEK293 cells. The luciferase expression level from the CRE reporter is used to monitor the activity of PDE7A in the cells.
询价背景介绍
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)
have been identified. PDE7 is widely expressed in various tissues, with PDE7A found
primarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7B
are detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads to
elevated intracellular level of cAMP.
产品介绍
Recombinant HEK293 cell line expressing human PDE7B (phosphodiesterase 7B, accession number NM_018945).
Human PDE7B has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7B expression was confirmed by Western blotting. The function of expressed PDE7B was validated by cAMP detection assay. The regulation of intracellular level of cAMP by PDE7B in PDE7B stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains the luciferase gene that is under the control of cAMP response element (CRE). Elevation of intracellular cAMP activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the luciferase reporter whereas hPDE7B-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase.
背景介绍
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE10A plays a key role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE10A hydrolyzes both cAMP and cGMP to nucleoside 5?-monophosphate, but exhibits higher affinity for cAMP, and it utilizes cAMP more efficiently as a substrate (Fujishige K. et al., 1999).
产品介绍
Recombinant HEK293 cell line expressing human PDE10A2 (phosphodiesterase 10A, accession number NM_006661).
N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.
背景介绍 The hedgehog pathway controls stem cell growth in embryonic and adult tissues and promotes tumor growth in a number of human cancers. The mammalian hedgehog proteins, Sonic Hedgehog (Shh), Indian Hedgehog (Ihh), and Desert Hedgehog (Dhh) activate hedgehog signaling by binding to their membrane receptor "Patched" (PTCH). This binding releases PTCH inhibition of Smoothened (Smo) and allows Smo to activate the Gli family of transcription factors, leading to transcription and expression of hedgehog signal target genes. 产品介绍 The Gli Reporter - NIH3T3 Cell Line is designed for monitoring the activity of the hedgehog signaling pathway. The Gli Reporter - NIH3T3 Cell Line contains the firefly luciferase gene under the control of Gli responsive elements stably integrated into NIH3T3 cells. Luciferase expression correlates with activation of the hedgehog signaling pathway.
询价背景介绍
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)
have been identified. PDE7 is widely expressed in various tissues, with PDE7A found
primarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7B
are detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads to
elevated intracellular level of cAMP.
产品介绍
Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A, accession number NM_031080).
N-terminal FLAG-tagged rat PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line and its expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by rat PDE7A in rat PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of luciferase reporter, whereas rat PDE7A-HEK293 cells showed reduction in the level of cAMP, resulting in lowered levels of luciferase expression. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.
背景介绍
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE7 is a cAMP-specific enzyme and two PDE genes (PDE7A and PDE7B)
have been identified. PDE7 is widely expressed in various tissues, with PDE7A found
primarily in skeletal muscle, T lymphocytes, and pancreas, while high levels of PDE7B
are detected in brain, heart, and liver. Inhibition of PDE7 activity by its inhibitors leads to
elevated intracellular level of cAMP.
产品介绍
Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603).
N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells, inducing the expression of the luciferase reporter, whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity.
背景介绍 The MAPK/ERK signaling pathway is a major participant in the regulation of cell growth and differentiation. It can be activated by various extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, MEK1/2 phosphorylate and activate ERK1/2. The activated ERK translocates to the nucleus where it phosphorylates and activates transcription factors. The TCFs (Ternary Complex Factors), including Elk1, are among the best-characterized transcription factor substrates of ERK. When phosphorylated by ERK, Elk1 forms a complex with Serum Response Factor (SRF) and binds to Serum Response Element (SRE), resulting in the expression of numerous mitogen-inducible genes. 产品介绍 The SRE Reporter - HEK293 cell line is designed for monitoring the activity of the JAK/STAT signaling pathway. The SRE Reporter - HEK293 cell line contains a firefly luciferase gene under the control of SRE responsive elements stably integrated into HEK293 cells, resulting in an ERK pathway-responsive reporter cell line.
询价背景介绍 The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins incluces mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. cJun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription. 产品介绍 The AP-1 Reporter - HEK293 Cell Linee is designed for monitoring the activity of the JNK signaling pathway. The AP1 Reporter - HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells.
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