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背景介绍
Phosphodiesterases (PDEs) regulate the intracellular levels of cAMP and cGMP by
hydrolyzing cAMP and cGMP to their inactive 5' monophosphates. These cyclic
nucleotides play an important role as second messengers in diverse physiological
functions. PDE1B plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. PDE1B hydrolyzes both cAMP and cGMP to nucleoside 5-prime monophosphate. Although it prefers cGMP at low substrate levels, it has a Vmax that is approximately equal for both cGMP and cAMP (Polli, J.W. and Koncaid, R.L., 1994; Yu, J. et al., 1997).
产品介绍
Recombinant HEK293 cell line expressing human PDE1B (phosphodiesterase 1B, accession number NM_000924).
N-terminal His-tagged human PDE1B has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of PDE1B was characterized by a cell-based cGMP assay. In this assay system, cells co-express atrial natriuretic peptide (ANP) receptor A (a guanylate cyclase), cyclic nucleotide5gated cation channel CNGA2, and the photoprotein aequorin. Intracellular cGMP level is monitored via aequorin luminescence induced by Ca2+ influx through CNGA2, acting as the intracellular cGMP sensor.
背景介绍 Biotin-labeling is commonly used for non-radioactive labeling and purification of proteins and other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) and either avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions of a protein and ligand, exhibiting a dissociation constant (Kd) around 10-15. BirA protein ligase covalently adds biotin to biotin-acceptor peptides containing an AviTag™ sequence in a highly efficient and specific ATP-dependent reaction. 产品介绍 BirA-transformed Competent E. coli cells are supplied as 10 x 50 µl vials. Strain BL21, a chemically competent E. coli B strain, contains an IPTG-inducible BirA expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene. These cells are compatible with most cloning vectors and are suitable for expression and biotinylation of recombinant proteins using AviTag™ technology.
询价产品介绍 Recombinant human angiopoietin-2 (Ang2), also known as Angpt2, expressed in HEK293 cells. Human Ang2 is a 70 kDa secreted ligand that binds the receptor Tie2.
询价产品介绍 Recombinant human angiopoietin-2 (Ang2), also known as Angpt2, expressed in HEK293 cells. Human Ang2 is a 70 kDa secreted ligand that binds the receptor Tie2.
询价产品介绍 Recombinant human vascular endothelial growth factor (VEGF)165 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF165 is the most common form of VEGF.
询价产品介绍 Recombinant human vascular endothelial growth factor (VEGF)165 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF165 is the most common form of VEGF.
询价产品介绍 Recombinant human vascular endothelial growth factor (VEGF)165 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF165 is the most common form of VEGF.
询价产品介绍 Recombinant human vascular endothelial growth factor (VEGF)121 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF121 is the smallest and most soluble form of VEGF.
询价产品介绍 Recombinant human vascular endothelial growth factor (VEGF)121 expressed in CHO cells. Human VEGF exists in multiple molecular variants and are named based on the number of amino acids they contain. VEGF121 is the smallest and most soluble form of VEGF.
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